Figure 5.

JE2ΔmpsABC has less α- and more ß-glycosylated GlcNAc WTA. Flow cytometry analysis of GlcNAc WTA (N-acetylglucosamine wall teichoic acid). Cells were grown in ambient air (A) or 5% CO₂ (CO₂) overnight, adjusted to A₅₇₈ 0.4 and treated with 200 µg/mL proteinase K. Isotype control was anti-HIV protein gp120 (b12-IgG) Fab fragment (5 μg/mL). Secondary antibody was fluorescein isothiocyanate-labeled goat anti-human IgG F(ab′)2 FITC conjugate (2 μg/mL). (a) mAb (monoclonal antibody) 4461 Fab fragment against TarM dependent α-GlcNAc WTA. (b) mAb 4497 Fab fragment against TarS dependent ß-GlcNAc WTA. MFI: mean fluorescence intensity. Each value in the graph shows the mean ± standard error of the mean (SEM) from at least three independent biological replicates. Significance was calculated by two-way ANOVA with (**** p < 0.05).