FIG. 3.

Induction of protective immunity against vaginal infection with HSV following mucosal genetic cotransfer of chemokines and gB DNA. Each group of mice (n = 14) coimmunized i.n. with gB DNA plus chemokine DNA was challenged intravaginally with HSV-1 McKrae 2 weeks the last immunization. Mice were previously injected with DP to synchronize their estrus cycles. Mice immunized i.n. with HSV-1 KOS (10⁶ PFU) were included as positive controls. (A) The surviving mice were counted 8 and 12 days following vaginal challenge. (B) Clinical severity was graded as follows: 0, no inflammation; 1, mild inflammation; 2, moderate swelling and redness; 3, severe inflammation; 4, paralysis; 5, death.