Table 2.
Plasmids used in the study.
| Plasmids | Relevant Characteristics | Sources |
|---|---|---|
| pBAD33 | Vector containing pBAD promoter with a p15A origin of replication (CmR) | [41] |
| pBbpfD | Sequence coding for BpfD (SO_4323) cloned into pBAD33 | This work |
| pBpdgA | Sequence coding for PdgA (SO_4552) cloned into pBAD33 | [24] |
| pBpdgB | Sequence coding for PdgB (SO_0796) cloned into pBAD33 | [24] |
| pBcheY3 | Sequence coding for CheY3 (SO_3209) cloned into pBAD33 | [20] |
| pET52b | Vector containing the T7 phage promoter and the coding sequence of StrepTagII (ApR) | Novagen |
| pETbpfD | Sequence coding for the cytoplasmic part (S225 to E639) of BpfD (SO_4323) cloned into pET52b | This work |
| pETbpfDGGDEF | Sequence coding for the GGDEF domain (S225 to T396) of BpfD (SO_4323) cloned into pET52b | This work |
| pETbpfDEAL | Sequence coding for the EAL domain (E397 to E639) of BpfD (SO_4323) cloned into pET52b | This work |
| pETcheY3 | Sequence coding for CheY3 (SO_3209) cloned into pET52b | [24] |
| pETpdgB | Sequence coding for PdgB (SO_0796) into pET21b | [24] |
| pEB355 | pUT18C derivative, coding for the T18 domain of the adenylate cyclase of Bordetella pertussis | [37] |
| pUT18-bpfD | Sequence coding for the cytoplasmic part (S225 to E639) of BpfD (SO_4323) cloned in frame at the 3’ extremity of the sequence coding for the T18 domain into pEB355 | This work |
| pUT18-zip | Sequence coding for a leucine zipper region cloned in-frame with the T18 domain (positive control) | [37] |
| pEB354 | pKT25 derivative, coding for the T25 domain of the adenylate cyclase of B. pertussis | [37] |
| pKT25-SO4324 | Sequence coding for SO_4324 cloned in frame at the 3’ end of the sequence coding for the T25 domain into pEB354 | This work |
| pKT25-cheY3 | Sequence coding for CheY3 (SO_3209) cloned in frame at the 3’ end of the sequence coding for the T25 domain into pEB354 | [24] |
| pKT25-pdgA | Sequence coding for PdgA (SO_4552) cloned in frame at the 3’ end of the sequence coding for the T25 domain into pEB354 | [24] |
| pKT25-pdgB | Sequence coding for PdgB (SO_0796) cloned in frame at the 3’ end of the sequence coding for the T25 domain into pEB354 | [24] |
| pKT25-zip | Sequence coding for a leucine zipper region cloned in-frame with the T25 domain (positive control) | [37] |
| pBAD24 | Vector containing pBAD promoter (ApR) | [41] |
| pBAD24-CBP-linker | Sequence coding for the calmodulin-binding protein (CBP) cloned into pBAD24 | [42] |
| pBcbp-bpfD | Sequence coding for the cytoplasmic part (S225 to E639) of BpfD (SO_4323) cloned in frame at the 3’ extremity of the sequence coding for the calmodulin-binding protein (CBP) into pBAD24-CBP-linker | This work |
| pKNG101 | R6K-derived suicide plasmid containing StrR and sacB | [39] |
| pRK2013 | RK2-Tra1 RK2-Mob1 KmR ori ColE1 | [43] |