Figure 7.

Stool parameters, GI transit and histological structure of colon in acetate-treated C3 KO mice. (A) Stool parameters. The preparation of the total stools was performed on three to five mice per group and each parameter was analyzed twice for each sample. (B) GI transit and total length of GI tract. After collection from the stomach to the anus, the entire GI tract was arranged in a row, and the location of the charcoal meal was marked with arrows. Transit ratio of charcoal meal and total length of GI tract were measured as described in materials and method. The preparation of the GI tract was performed on three to five mice per group, and each parameter was analyzed twice for each mouse. (C) Histological structure of mid colon. The H&E-stained tissue sections were prepared from tissue samples from three to five mice per group, and the pathological factors were analyzed twice for each stained tissue. The data are presented as the mean ± SD. * p < 0.05 vs. no treated group. # p < 0.05 vs. vehicle-treated C3 KO group. Abbreviation: WT, wild type; C3 KO, complement C3 knockout; ALPI, alkaline phosphatase intestinal; H&E, hematoxylin and eosin.