Figure 10.

Scheme for purification of epithelial cells from intestine of C3 KO mice and acetate treatment. After collection of the epithelial cells from the intestines of WT and C3 KO mice, these cells were treated with 5 mM acetate or 1× PBS, and the expression of several genes were evaluated by RT-qPCR analysis using specific primers. Abbreviation: WT, wild type; C3 KO, complement C3 knockout; ALPI, alkaline phosphatase intestinal; C-kit, receptor protein kinase; PBS, phosphate buffer saline; RT-qPCR; quantitative real-time PCR.