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. 2024 Sep 5;25(17):9627. doi: 10.3390/ijms25179627

Figure 2.

Figure 2

Formation of ECTs with select conditions is comparable to standard (RPMI/B27) ECTs, with extent of 2D compaction correlating with CSA and elastic modulus. (A) Conditions obtained from 2D screening results used for ECT testing. (B) Brightfield images illustrating tissue compaction from initial casting on day 0 (D0) to day 7 (D7) of in vitro culture (mold size, 3 × 9 mm). (C) Survival curve showing percentage of intact ECTs to assess structural survival. (D) Quantification of tissue compaction over 7-day culture; (E) Quantification of tissue cross sectional area at D7 of culture. (F) Elastic modulus for ECTs shows functional stiffness of tissues. n = 6–17 samples per group, with significance defined as ** p < 0.01; *** p < 0.001, **** p < 0.0001. # represents significance between all groups except RPMI vs. FA; RPMI vs. RPMI-HPC; MM vs. MM-HPC; FA vs. RPMI-HPC; and FA vs. MM-HPC. $ represents significance between RPMI vs. FA and FA vs. RPMI-HPC; & represents significance between RPMI vs. MM; MM vs. FA; MM vs. RPMI-HPC; and RPMI-HPC vs. MM HPC. % represents significance between RPMI vs. RPMI-HPC and FA vs. RPMI-HPC. @ represents significance between RPMI vs. MM; RPMI vs. MM-HPC; MM vs. FA; and RPMI-HPC vs. MM HPC.