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. 2024 Sep 5;25(17):9626. doi: 10.3390/ijms25179626

Figure 6.

Figure 6

Figure 6

STAT3 inhibition attenuates Aβ25-35-mediated PKCδ induction, p35 cleavage into p25, neuronal CCR, and caspase-3 cleavage in the post-mitotic neurons. (A–C) Primary cortical neurons were exposed to 10 μM Aβ25-35 with or without 5 μM stattic for 24 h before detection of STAT3 (A), PKCδ (B), or PCNA (C). (D–H) Primary cortical neurons were transfected with LacZ-shRNA or STAT3-shRNA for 24 h, followed by exposure to 10 μM Aβ25-35 for an additional 8 h, before detection of p35/p25 (F) or 24 h before detection of STAT3 (D), PKCδ (E), and PCNA (G), as well as pro- and cleaved caspase 3 (H). β-Actin and α-tubulin served as the internal control for equal loading of proteins in each lane. Mean ± S.E.M. from N = 3 in (A), N = 4 in (B,C), N = 3 in (D), N = 4 in (E), and N = 3 in (F–H). *, #, and + all denote p < 0.05.