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. 2024 Sep 5;25(17):9626. doi: 10.3390/ijms25179626

Figure 7.

Figure 7

Aβ25-35-induced calpain2, but not calpain1, mediates PKCδ-dependent p35 cleavage into p25, neuronal CCR, and caspase-3 cleavage. (A) Primary cortical neurons were transfected with Luc-shRNA or PKCδ-shRNA for 24 h, followed by exposure to 10 μM Aβ25-35 for an additional 8 h, before detection of calpain2. (B-E) Primary cortical neurons were transfected with Luc-shRNA or calpain2-shRNA (shCapn2) for 24 h, followed by exposure to 10 μM Aβ25-35 for an additional 8 h (B–D) or 24 h (E), before detection of calpain2 (B), p35/p25 (C), and cyclin D1 (D), as well as pro- and cleaved caspase-3 (E). (F) Primary cortical neurons were transfected with Luc-shRNA or PKCδ-shRNA for 24 h, followed by exposure to 10 μM Aβ25-35 for an additional 8 h, before detection of calpain1. (G–J) Primary cortical neurons were transfected with Luc-shRNA or calpain1-shRNA (shCapn1) for 24 h, followed by exposure to 10 μM Aβ25-35 for an additional 8 h (G–I) or 24 h (J), before detection of calpain1 (G), p35/p25 (H), and cyclin D1 (I), as well as pro and cleaved caspase 3 (J). β-Actin and α-tubulin served as the internal control for equal loading of proteins in each lane. Mean ± S.E.M. from N = 5 in (A), N = 4 in (B, C), N = 3 in (D, E), N = 4 in (F), and N = 3 in (G-J). *, #, and + all denote p < 0.05; “ns” denotes “not significant”.