Figure 8.

Aβ25-35 induces PKCδ- and CDK5-dependent expression of PUMA. (A,B) Primary cortical neurons were transfected with PKCδ-shRNA (A) or CDK5-shRNA (B) for 24 h, followed by exposure to 10 μM Aβ25-35 for an additional 24 h, before detection of PUMA by Western blotting. Cortical cultures transfected with LacZ-shRNA served as the negative control for lentivirus-mediated gene transfer. β-Actin served as the internal control for equal loading of proteins in each lane. Mean ± S.E.M. from N = 3. * and # denote p < 0.05.