FIG. 6.

CTLA4Ig treatment causes anergy in HSV-specific CD4⁺ T cells but not in CD8⁺ T cells. Day 5 draining LN cells from CD154^−/− mice (), CTLA4Ig-treated mice (■), and corresponding controls () were analyzed directly ex vivo or after 3 days in culture with or without IL-2 (5 ng/ml) for HSV-specific IFN-γ production in response to UVHSV or HSVgB_498–505. Plots represent fractions of IFN-γ-producing HSV-specific CD4⁺ or CD8⁺ cells in large CD4⁺ or CD8⁺ populations in CD154^−/− (a) or CTLA4Ig-treated (b) mice.