Fig. 4.

Effect of baicalin with different concentrations on transcriptional activity of NF-κB after exposure of HMEC to CAC. HMEC were inoculated into 96-well plates overnight and then transfected with NF-κB expression plasmid and liposome reagents. The cells were then pretreated with different concentrations (10^− 10, 10^− 11, and 10^− 12 M) for 2 h and incubated with 30 µL of CAC product for 4 h. Luciferase activity was measured using a dual luciferase reporter gene assay system, and values represent the mean ± SD (n = 8). **P < 0.01 compared with control group; ^##P < 0.01 compared with model group