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. 2024 Sep 13;24:970. doi: 10.1186/s12879-024-09869-x

Table 2.

PCR thermal cycling conditions [43]

Gene Initial denaturation No of cycles Denaturation Annealing Extension Final extension
OXA-48 94 °C for 10 min 36 cycles 94 °C for 30 s 52 °C for 40 s 72 °C for 50 s 72 °C for 5 min
NDM-1 94 °C for 10 min 36 cycles 94 °C for 30 s 52 °C for 40 s 72 °C for 50 s 72 °C for 5 min