FIG. 1.

Isolation of protease mutant procapsids by precipitation with a VP5-specific MAb. (A) Coomassie blue-stained SDS-PAGE gel comparing the compositions of sucrose gradient-purified B-capsids with m100 procapsids isolated by precipitation with MAb 6F10. Results for twofold dilutions of B-capsids and procapsids are shown. The positions of antibody heavy (IgH) and light (IgL) chains, capsid proteins, and cellular actin are indicated. A mock-infected cell lysate, treated identically to the 6F10 MAb, is shown as a control. The amount of sample loaded in the “mock” lane is equivalent to the largest amount of m100 procapsids loaded on the gel. (B) Coomassie blue-stained SDS-PAGE gel demonstrating the MAb precipitation technique does not isolate capsid proteins from a ts1178 (capsid-minus) extract. Two different antibody-isolated preparations of m100 procapsids are shown. Note also the comparison of the protein composition of sucrose-gradient purified HSV-1(KOS) B- and C-capsids with that of procapsids isolated from m100 infected cells.