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. 2001 Jan;75(2):687–698. doi: 10.1128/JVI.75.2.687-698.2001

TABLE 2.

UL25 protein levels are lowest in procapsids and highest in C-capsids

Capsid type % of UL25 protein relative to B-capsidsa
Expt. 1 Expt. 2 Expt. 3 Expt. 4 Mean
Procapsidb 19 17 25 ND 20
B-capsid 100 100 100 100 100
C-capsid 297  NDc 258 387 314
a

Protein levels were determined by densitometric scanning of film exposures of Western blots, as described in Materials and Methods. The results are presented as the percentage of UL25 protein relative to HSV-1(KOS) B-capsids, examined in parallel. Comparisons between samples and experiments were performed by setting levels for the VP5 protein to 100%. 

b

Procapsids were isolated from m100-infected cells (experiments 1 and 2) or tsProt.A-infected cells (experiment 3). B- and C-capsids were isolated from HSV-1(KOS)-infected cells (experiments 1 to 4). 

c

ND, not done.