TABLE 2.
UL25 protein levels are lowest in procapsids and highest in C-capsids
Capsid type | % of UL25 protein relative to B-capsidsa
|
||||
---|---|---|---|---|---|
Expt. 1 | Expt. 2 | Expt. 3 | Expt. 4 | Mean | |
Procapsidb | 19 | 17 | 25 | ND | 20 |
B-capsid | 100 | 100 | 100 | 100 | 100 |
C-capsid | 297 | NDc | 258 | 387 | 314 |
Protein levels were determined by densitometric scanning of film exposures of Western blots, as described in Materials and Methods. The results are presented as the percentage of UL25 protein relative to HSV-1(KOS) B-capsids, examined in parallel. Comparisons between samples and experiments were performed by setting levels for the VP5 protein to 100%.
Procapsids were isolated from m100-infected cells (experiments 1 and 2) or tsProt.A-infected cells (experiment 3). B- and C-capsids were isolated from HSV-1(KOS)-infected cells (experiments 1 to 4).
ND, not done.