FIG. 3.

Glycoprotein composition of mutant virions. Serial twofold dilutions of wild-type virions or virions lacking gB, gC, gD, or gH were subjected to SDS-PAGE, the electrophoresis products were transferred to nitrocellulose, and the blots were probed for virion glycoproteins. Mutant and wild-type virions were compared on individual gels, and in each instance the undiluted sample (left lane) contained approximately 5 μg of protein. VP16 was simultaneously detected as an internal control for virion load. Where gD was detected, gB or gC was simultaneously detected as a loading control. Thus, in series A, the left panel shows that the VP16 loads for wild-type and gH-negative virions are similar and that the gB content is also similar. In the center panel, which compares the gD content, gB is therefore used as an internal control for loading. Series A, comparison of wild-type and gH-negative virions; series B, comparison of wild-type and gC-negative virions; series C, comparison of wild-type and gB-negative virions; series D, comparison of wild-type and gD-negative virions.