Skip to main content
. 2001 Jan;75(2):726–737. doi: 10.1128/JVI.75.2.726-737.2001

FIG. 3.

FIG. 3

Identification of ALV(A) variants with env mutations. The SU regions of the env genes of ALV proviruses in genomic DNA isolated from each infected culture were amplified by PCR and cloned as KpnI-SalI fragments, and the nucleotide sequences were determined. A schematic of the cloned region is included at the top of the figure. The amino acids are numbered from the start of the mature SU glycoprotein (gp85). The amino acids contained in the five variable regions are vr1, 64 to 75; vr2, 100 to 105; hr1, 122 to 165; hr2, 199 to 227; and vr3, 261 to 269. The deduced amino acid sequences of the hr1 and hr2 regions of SU cloned from the sTva-mIgG-selected viral population (clones 1 to 24) and the control (unselected) viral population (clones C1 to C19) are shown. Clones 1 to 12 and C1 to C9 were amplified by Taq DNA polymerase, whereas clones 13 to 24 and C10 to C19 were amplified by Vent DNA polymerase. Only the differences in amino acid sequence compared to SR-A SU (WT) are shown. Additional amino acid changes in this region of SU are listed for each clone.