Table 2.

Performance comparison between (i) NHM and BMC qPCR methods and (ii) between the two qPCRs and microscopy in field-collected stool samples

	Two qPCRs—BCM and NHM (two raters)			Fleiss kappa (three raters)
	Unweighted Cohen’s kappa agreement	Percentage agreement	P-value	Fleiss kappa (categorical)	P-value
Necator americanus	0.33	82	< 0.01	0.21	< 0.01
Trichuris trichiura	0.37	96	< 0.01	0.18	< 0.01
Ancylostoma duodenale	0.28	64	< 0.001	0.15	< 0.01
Strongyloides stercoralis	0.12	77	< 0.001	0.06	0.02
Ascaris lumbricoides	0.45	92	< 0.001	0.22	< 0.01

Cohen’s and Fleiss kappas were calculated to evaluate the agreement between the two qPCR assays (two raters) and the agreement between microscopy and the two qPCR tests (three raters), respectively, treating results as categorical values (presence/absence). Kappa < 0 means no agreement; 0–0.20 slight agreement; 0.21–0.40 fair; 0.41–0.60 moderate; 0.61–0.80 substantial; and 0.81–1.0 perfect. All calculations for kappa (R package irr) and visuals for the correlations were conducted in R v.4.2.2