Table 4.
Conditions for RT-qPCR.
| Gene | Annealing | Extension | ||
|---|---|---|---|---|
| Temperature | Time | Temperature | Time | |
| °C | s | °C | s | |
| 18S 1 | 60 | 30 | 72 | 8 |
| C3H 2 | 58 | 30 | 72 | 9 |
| FLS 3 | 56 | 30 | 72 | 9 |
1 Reference gene; 2 coumarate 3-hydroxylase enzyme gene; 3 flavonol synthase enzyme gene.
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