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. 2024 Sep 3;13(17):2466. doi: 10.3390/plants13172466

Table 5.

Sequence of the primers used for real-time relative quantification.

Gene 5′→3′ 3′→5′ Length
bp
18S 1 GCC TAC TAT GGT GGT GAC GG CTA CCT CCC CGT GTC AGG AT 129
C3H 2 CAA GAA GAG CTC GAC CGT GT TTG CAT TGG CTT TGT GTG GG 148
FLS 3 CCA TAC AGA ATA TGT CCT CCA TCA CC GCT CAA TAT GTC CAT TTG GTC ACC 146

1 Reference gene; 2 coumarate 3-hydroxylase enzyme gene; 3 flavonol synthase enzyme gene.