Generation and functional analysis of GhPEL48_Dt transgenic Arabidopsis. (A) Semi-quantitative PCR of GhPEL48_Dt transgenic Arabidopsis. (B) Relative expression levels of GhPEL48_Dt in wild-type and Arabidopsis overexpressing GhPEL48_Dt. (C) Pectate lyase activity of wild-type and GhPEL48_Dt overexpressing Arabidopsis. (D) The phenotype of trichomes in wild-type and GhPEL48_Dt transgenic Arabidopsis. (E) Statistical analysis of trichome density in wild-type and GhPEL48_Dt transgenic Arabidopsis. (F) The phenotypes of root hair in TSA-treated and untreated wild-type and GhPEL48_Dt transgenic Arabidopsis. (G) Statistical analysis of root hair length in GhPEL48_Dt transgenic Arabidopsis, TSA-treated and control. (H) Quantitative analysis of root hair density in GhPEL48_Dt transgenic Arabidopsis, TSA-treated and control. Gray box represents control; black box represents lines treated with TSA. Asterisks in figureB, C and E indicate a significant difference (Student′s t; ns p > 0.05, * p < 0.05, *** p < 0.001). Letters above the bars (in (G,H)) indicate signifcant diference according to one-way ANOVA analysis at p < 0.05.