Table 1. Sequence of the primers and TaqMan probe for the detection of the E6 gene of HPV-16 and HPV-18.
Oligonucleotides | Sequence (5’-3’) | qPCR product size |
---|---|---|
Oligonucleotides for HPV-16 | 128 bp | |
HPV-16 For | GAACCGAAACCGGTTAGTATAA | |
HPV-16 Rev | ATGTATAGTTGTTTGCAGCTCTGT | |
HPV-16 p | 6HEX-CATTTTATGCACCAAAAGAGAACTGCAATGTTTC-BHQ1 | |
Oligonucleotides for HPV-18 | ||
HPV-18 For | GGACCGAAAACGGTGTATATAA | 124 bp |
HPV-18 Rev | CAGTGAAGTGTTCAGTTCGGT | |
HPV-18 p | TAMRA-ATGTGAGAAACACACCACAATACTATGGCGCG-BHQ2 |