Tabla 2. Programa de ciclaje para la qPCR de VPH.
| Ciclaje | |||
|---|---|---|---|
| Paso | Temperatura | Tiempo | Nº de ciclos |
| Activación inicial | 95 ºC | 15 min | 1 |
| Denaturación | 94 ºC | 5 s | 45 |
| Hibridación | 50 ºC | 20 s | |
| Extensión | 60 ºC | 40 s | |
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