TABLE 2.
Cell surface distribution of enveloped HSV-1 on the surfaces of MDBK cellsa
| Virus | No. of virus particles per cellb
|
|||
|---|---|---|---|---|
| Apical | Basal | Cell junctions | Total | |
| Wild type | 168 (3.3) | 653 (12.8) | 2,101 (41.2) | 2,922 (57.3) |
| F-gEβ | 611 (10.0) | 334 (5.5) | 116 (1.9) | 1,061 (17.4) |
| F-gEΔCT | 653 (11.9) | 158 (2.9) | 66 (1.2) | 877 (15.9) |
a
MDBK cells growing on Thermanox plastic coverslips were infected with wild-type HSV-1 strain F, F-gEβ, or F-gEΔCT for 16 h. The cells were then fixed and processed for electrom microscopy as described for Table 1.
b
The total numbers of particles on apical or basal surfaces or at cell junctions are shown, and the numbers in parentheses are the average numbers of particles per cell.