TABLE 3.

Distribution of virus particles in polarized MDBK and nonpolarized Vero cells^a

Cells	Intracellular location	Virion structure	No. of particles/cellb
			Wild-type HSV-1	F-gEβ
MDBK	Nucleus	Nonenveloped	27 ± 3.9	19 ± 5.2
	Cytoplasm	Enveloped	23 ± 3.0	111 ± 7.7
		Nonenveloped	3.2 ± 2.6	7.1 ± 4.1
	Cell surface	Enveloped	88 ± 9.7	20 ± 3.8
Vero	Nucleus	Nonenveloped	35 ± 2.6	37 ± 3.4
	Perinuclear space	Enveloped	18 ± 4.1	12 ± 4.2
	Cytoplasm	Enveloped	31 ± 4.2	35 ± 2.1
		Nonenveloped	5.2 ± 2.4	6.4 ± 3.2
	Cell surface	Enveloped	102 ± 6.7	89 ± 8.6

^aMDBK or Vero cells growing in plastic dishes were infected with wild-type HSV-1 strain F or F-gEβ for 20 h. Cells were then fixed with glutaraldehyde, scraped from the plastic, and processed for electron microscopy. Approximately 2,200 wild-type virus particles and 2,200 F-gEβ particles were counted in the MDBK cell samples. 

^bData are averages with standard deviations.