Table 1:

Quantification of drug synergy for two cell lines (MCF7, T-47D) when confronted to two drugs: chemotherapy (paclitaxel or doxorubicin) in combination with disulfiram. Cell lines are either naive, or evolved resistance to paclitaxel or doxorubicin. Each dose response checkerboard assay is repeated four times (batch numbers 1–4 are related to different concentrations of disulfiram, in increasing concentrations) and synergy is measured using the MUSYC software package^11,12. We consider the effect of disulfiram on the EC50 (potency; log(a₂₁): see Methods) and on maximal response (efficacy; 𝛽: see Methods)

Cell line	Drug 1	Drug 2	Type	Batch	Potency	Log(a21)	Efficacy	𝛽
MCF7	Doxo	Disu	Naive	1	Neutral	0.03 (−0.27, 0.36)	Synergy	0.05 (0.04, 0.19)
				2	Neutral	−0.01 (−0.29, 0.29)	Neutral	−0.22 (−0.89, 0.22)
				3	Neutral	−0.14 (−0.46, 0.15)	Synergy	0.07 (0.04, 0.22)
				4	Neutral	0.06 (−0.32, 0.35)	Synergy	0.05 (0.01, 0.19)
MCF7	Doxo	Disu	DoxoR	1	Neutral	2.6 (−0.18, 4.76)	Synergy	0.34 (0.24, 0.82)
				2	Synergy	1.5 (0.39, 2.73)	Synergy	0.29 (0.2, 0.6)
				3	Synergy	1.1 (0.53, 2.18)	Synergy	0.44 (0.32, 0.8)
				4	Synergy	4.2 (1.00, 7.06)	Synergy	0.46 (0.32, 1.01)
MCF7	Doxo	Disu	PaclR	1	Neutral	1.1 (−0.62, 2.53)	Synergy	0.19 (0.08, 0.41)
				2	Synergy	1.4 (0.05, 2.66)	Synergy	0.19 (0.11, 0.48)
				3	Neutral	0.8 (−0.14, 2.11)	Neutral	−0.23 (−0.93, 0.3)
				4	Synergy	1.8 (0.78, 3.76)	Antagonism	−0.28 (−0.33, −0.06)
MCF7	Pacl	Disu	Naive	1	Neutral	−0.2 (−0.56, 0.69)	Synergy	0.19 (0.17, 0.22)
				2	Neutral	0.5 (−0.55, 3.26)	Synergy	0.15 (0.13, 0.19)
				3	Neutral	−0.2 (−0.62, 0.51)	Synergy	0.15 (0.13, 0.2)
				4	Neutral	−0.1 (−0.61, 0.6)	Synergy	0.18 (0.16, 0.26)
MCF7	Pacl	Disu	PaclR	1	Neutral	4.6 (−0.17, 4.59)	Synergy	0.08 (0.05, 0.21)
				2	Neutral	−0.6 (−4.62, 3.38)	Neutral	0.06 (−0.03, 0.19)
				3	Neutral	1.8 (−0.18, 5.75)	Synergy	0.21 (0.14, 0.45)
				4	Neutral	6.8 (0.00, 8.00)	Neutral	0.03 (0.00, 0.13)
MCF7	Pacl	Disu	DoxoR	1	Neutral	−6.6 (−8.00, 0.00)	Neutral	0.01 (−0.14, 0.07)
				2	Neutral	0.8 (−0.56, 1.39)	Neutral	0.00 (−0.02, 0.08)
				3	Neutral	0.0 (−8, 0.00)	Synergy	0.06 (0.03, 0.1)
				4	Neutral	7.0 (0, 7.98)	Neutral	−0.01 (−0.02, 0.08)
T-47D	Doxo	Disu	Naive	1	Neutral	−0.2 (−0.44, 0.06)	Neutral	−0.34 (−0.48, 0.11)
				2	Neutral	−0.3 (−0.53, 0.11)	Neutral	−0.16 (−0.98, 0.14)
				3	Neutral	−0.3 (−0.57, 0.02)	Neutral	0.10 (−0.99, 0.16)
				4	Neutral	−0.3 (−0.60, 0.20)	Neutral	−0.09 (−0.96, 0.13)
T-47D	Doxo	Disu	DoxoR	1	Neutral	0.5 (−0.18, 1.47)	Synergy	0.10 (0.04, 0.31)
				2	Neutral	0.3 (−0.32, 1.24)	Synergy	0.11 (0.07, 0.26)
				3	Neutral	−0.2 (−3.96, 1.09)	Synergy	0.21 (0.14, 0.29)
				4	Neutral	−0.1 (−4.12, 1.38)	Synergy	0.12 (0.03, 0.21)
T-47D	Doxo	Disu	PaclR	1	Neutral	0.7 (−0.5, 1.82)	Neutral	0.02 (−0.01, 0.09)
				2	Neutral	−1.1 (−5.1, 0.71)	Neutral	0.05 (−0.03, 0.09)
				3	Neutral	0.3 (−0.31, 0.86)	Synergy	0.03 (0.02, 0.08)
				4	Synergy	0.7 (0.15, 1.26)	Synergy	0.04 (0.02, 0.1)
T-47D	Pacl	Disu	Naive	1	Antagonism	−0.5 (−0.82, −0.13)	Synergy	0.05 (0.05, 0.08)
				2	Neutral	−0.5 (−0.9, 0.24)	Synergy	0.04 (0.03, 0.06)
				3	Neutral	−0.3 (−0.68, 0.64)	Synergy	0.03 (0.02, 0.06)
				4	Neutral	4.0 (0.00, 8.00)	Synergy	0.05 (0.05, 0.08)
T-47D	Pacl	Disu	PaclR	1	Neutral	−1.3 (−5.22, 0.9)	Neutral	−0.01 (−0.03, 0.04)
				2	Neutral	8.0 (0.00, 8.00)	Neutral	0.01 (0.00, 0.06)
				3	Neutral	8.0 (0.00, 8.00)	Neutral	0.00 (−0.01, 0.05)
				4	Neutral	0.5 (−3.15, 4.85)	Neutral	0.00 (−0.01, 0.07)
T-47D	Pacl	Disu	DoxoR	1	Neutral	−0.07 (−4.03, 2.16)	Synergy	0.03 (0.02, 0.1)
				2	Synergy	6.6 (0.8, 6.35)	Neutral	0.00 (−0.01, 0.07)
				3	Synergy	0.8 (0.38, 1.27)	Neutral	0.01 (−0.01, 0.07)
				4	Synergy	2.6 (0.9, 4.94)	Neutral	0.01 (−0.02, 0.07)