KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-FLAG M2 antibody | Sigma-Aldrich | F3165 |
| Anti-HA antibody | Sigma-Aldrich | H3663 |
| Anti-HPIV3-N | This study | N/A |
| Anti-HPIV3-M | This study | N/A |
| Anti-eIF4Alll | Abcam | Ab180573 |
| Anti-Y14 | Abcam | Ab181038 |
| Anti-MAGOH | Abcam | Ab180505 |
| Anti-GFP | Cell Signaling | 2956 |
| Anti-S6 Ribosomal Protein | Cell Signaling | 2217, 2317 |
| Anti-L7a Ribosomal Protein | Cell Signaling | 2415 |
| Anti-GAPDH | Cell Signaling | 2118 |
| Anti-β-Actin | Cell Signaling | 3700 |
| Anti-β-Tubulin | Cell Signaling | 2128 |
| Anti-Lamin A/C | Cell Signaling | 4777 |
| Anti-COX IV | LI-COR | 926-42214 |
| Anti-Enterovirus D68 VP1 | GeneTex | GTX125989 |
| Anti-Influenza A Virus Nucleoprotein | GeneTex | GTX125989 |
| Anti-SARS-CoV2-N | Dr. Thomas Moran | Clone:1C7 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | ThermoFisher | A21245 |
| Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | ThermoFisher | A21236 |
| Bacterial and virus strains | ||
| Recombinant rHPIV3-EGFP JS strain | PMID: 28405630 | GenBank: KY295925 |
| Recombinant rHPIV3-EGFP-HA-M JS strain | This study | N/A |
| Recombinant rHPIV3-EGFP-ΔM-mCherry JS strain | This study | N/A |
| Recombinant rHPIV3-EGFP-ΔM JS strain | This study | N/A |
| Recombinant rMuV-EGFP JL5 strain | PMID: 28405630 | GenBank: KY295913 |
| Recombinant rNDV-EGFP LaSota strain | PMID: 28405630 | GenBank: KY295917 |
| Recombinant rCedar-EGFP CG1a strain | This study | GenBank: JQ001776 |
| EV D68 strain US/MO/14-18947 | ATCC | VR1283 |
| Influenza A virus strain A/WSN/33 | PMID: 561860 | N/A |
| SARS-CoV-2 strain USA/WA1 | BEI resources | NR-52281 |
| Max Efficiency Stbl2 competent cells | ThermoFisher | 10268019 |
| Chemicals, peptides, and recombinant proteins | ||
| DMEM, high glucose, pyruvate | ThermoFisher | 11995-065 |
| Opti-MEM, Reduced Serum Medium | ThermoFisher | 31985-070 |
| Lipofectamine LTX and PLUS reagent | ThermoFisher | 15338100 |
| Lipofectamine RNAiMAX Transfection Reagent | ThermoFisher | 13778100 |
| BioT transfection reagent | Bioland Scientific | B01-01 |
| Fetal Bovine Serum - Optima | R&D Systems | S12450 |
| Fetal Bovine Serum - Tetracycline-free) | Takara | 631101 |
| Penicillin-Streptomycin | ThermoFisher | 15140122 |
| PBS | ThermoFisher | 10010023 |
| Trypsin-EDTA (0.25%) | ThermoFisher | 2500-056 |
| InFusion HD Cloning Kit | Takara Bio | 639650 |
| Paraformaldehyde 16% Aqueous Solution | Electron Microscopy Sciences |
15710-S |
| Intercept Blocking Buffer | Li-COR | 927-70001 |
| Collagen I-coated coverslip | Neuvitro | GG-18-15-Collagen |
| Cycloheximide (CHX) | Sigma-Aldrich | C7698-1G |
| Triton X-100 | Sigma-Aldrich | T8787 |
| Diethiothreitol (DTT) | Sigma-Aldrich | D9779 |
| Puromycin | ThermoFisher | A1113803 |
| Zeocin | ThermoFisher | R25001 |
| 4–15% Mini-PROTEAN TGX Precast Protein Gels | Bio-Rad | 4561083,4561086 |
| Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit | Bio-Rad | 1704272 |
| ProLong Glass Antifade Mountant with NucBlue Stain | ThermoFisher | P36983 |
| 1M Tris-HCl pH 8.0 | ThermoFisher | AM9855G |
| 1M Tris-HCl pH 7.5 | ThermoFisher | 15567027 |
| 0.5M EDTA pH 8.0 | ThermoFisher | 15575020 |
| 5M NaCl | ThermoFisher | AM9760G |
| 1M MgCl2 | ThermoFisher | AM9530G |
| 2M KCl | ThermoFisher | AM9640G |
| RNaseOUT | ThermoFisher | 10777019 |
| Halt™ Protease Inhibitor Cocktail, EDTA-Free | ThermoFisher | 78437 |
| Trichloroacetic acid (TCA) | Sigma-Aldrich | T9159-100G |
| Anti-FLAG M2 affinity gel | Sigma-Aldrich | A2220 |
| Anti-HA Magnetic Beads | ThermoFisher | 88837 |
| Critical commercial assays | ||
| Direct-zol RNA miniprep kit | Zymo Research | R2050 |
| Luna Universal qPCR Master Mix | NEB | M3003 |
| LunaScript RT SuperMix Kit | NEB | E3010 |
| Nano-Glo HiBit lytic detection system | Promega | N3040 |
| ONE-Glo luciferase assay system | Promega | E6100 |
| Deposited data | ||
| Polysome profile mRNA sequencing data | This study | GSE274026 |
| Experimental models: Cell lines | ||
| HEK 293T | ATCC | Cat# CRL-3216 |
| HeLa | ATCC | Cat# CCL-2 |
| Vero | ATCC | Cat# CCL81 |
| Vero-E6 | ATCC | Cat# CRL-1586 |
| RD | ATCC | Cat# CCL-136 |
| Vero-HPIV3-Mpot | This study | N/A |
| BSR-T7 | PMID: 9847328 | N/A |
| BSR-T7-HPIV3-Mopt | This study | N/A |
| BSR-T7-rNiV-N-P-L replicon | This study | |
| Oligonucleotides | ||
| See Table S1 | This study | N/A |
| Recombinant DNA | ||
| pCMV-3Tag-NiV-M | PMID: 21085610 | N/A |
| pCMV-3Tag-HeV-M | PMID: 25782006 | N/A |
| pCMV-3Tag-GhV-M | PMID: 25782006 | N/A |
| pCMV-3Tag-SeV-M | PMID: 25782006 | N/A |
| pCMV-3Tag-MuV-M | PMID: 25782006 | N/A |
| pCMV-3Tag-MeV-M | PMID: 25782006 | N/A |
| pCMV-3Tag-HPIV2-M | PMID: 25782006 | N/A |
| pCMV-3Tag-NDV-M | PMID: 25782006 | N/A |
| pCMV-3Tag-HPIV3-M | This study | N/A |
| pCMV-3Tag-CedV-M | This study | N/A |
| pCMV-3Tag-GFP-NiV-M | PMID: 21085610 | N/A |
| pCMV-3Tag-GFP-NiV-M Mbp1/2 | PMID: 21085610 | N/A |
| pCMV-3Tag-GFP-NiV-M L106A, L107A | PMID: 21085610 | N/A |
| pFLAG-CMV2-GFP-HPIV3-M | This study | N/A |
| pFLAG-CMV2-GFP-HPIV3-M Mbp1/2 | This study | N/A |
| pFLAG-CMV2-GFP-HPIV3-M L106A, L107A | This study | N/A |
| pCAGGS-HPIV3-N | This study | N/A |
| pCAGGS-HPIV3-P | This study | N/A |
| pCAGGS-HPIV3-M | This study | N/A |
| pCAGGS-HPIV3-F | This study | N/A |
| pCAGGS-HPIV3-HN | This study | N/A |
| pCAGGS-HPIV3-L | This study | N/A |
| pCMV-LUC2CP/intron/ARE | Addgene | #62858 |
| pCW57.1 | Addgene | #41393 |
| pCW57.1 | ||
| Software and algorithms | ||
| Prism | GraphPad | Version 10 |
| SnapGene | SnapGene.com | Version 4.2.11 |
| Image Lab | Bio-Rad | Version 6.1 |
| Partek Flow | Partek | Version 10 |
| Imaris | Oxford Instruments | Version 9 |
| Celigo Imaging Software | Nexcelom | Version 5.5 |
| Other | ||
| Celigo Imaging Cytometer | Nexcelom | N/A |
| Gradient Mate Station | Biocomp | N/A |
| Piston gradient fractionator | Biocomp | N/A |
| EVOS M5000 | ThermoFisher | N/A |
| ChemiDoc MP Imaging system | Bio-Rad | N/A |
| CFX96 Real-Time PCR system | Bio-Rad | N/A |