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[Preprint]. 2024 Sep 5:2024.09.05.611469. [Version 1] doi: 10.1101/2024.09.05.611469

Fig. 3. The global transcriptional signatures from Nsp15 wild-type and mutant SARS-CoV-2 infected A549-ACE2 cells.

Fig. 3

A549-ACE2 cells with mock, Nsp15WT, Nsp15H234A, and Nsp15N277A rSARS-CoV-2 infection for 8 and 24 h (MOI of 5). Total RNA with poly(A) enrichment followed by RNA sequencing analysis. (A) Schematic of bulk RNA-seq experimental design (n = 3 per group). (B) PCA of total normalized transcript abundance from mock, Nsp15 wild-type and mutant rSARS-CoV-2 infection. Sparse PCA depicts the global transcriptome of individual sample. (C) Venn diagram for unique and shared differentially expressed genes (Padj < 0.05 & |log2FC| > 1) in cells infected with Nsp15H234A and Nsp15N277A mutants compared to Nsp15WT virus. (D) Volcano plots showing GSEA results generated using MSigDB Hallmark pathways. (E) Cluster heatmap of GSVA scores generated using representative innate immune and metabolic signatures from MSigDB Gene Ontology signatures. (F) Expression heatmap of representative innate immune and metabolic genes in across mock, wild-type, and mutant rSARS-CoV-2 infection.