Extended Data Fig. 4. Mutation of NR-box 2 or multiple NR-boxes to LXXAA in SRC3 reduces PR activity.

Bar graph showing the ratio of Firefly to Renilla luciferase normalized over the vehicle (ethanol) signal for both R5020 PR agonist and RU486 PR antagonist. Mock is indicative of base vector in the place of PR or SRC3 plasmid. ‘Mut’ in the legend indicates an LXXLL NR-Box mutated to LXXAA, validated by whole plasmid next-generation sequencing (Azenta). Similarly, ‘BOX’ denoted in the PR-A experiments indicates the mutated NR-Box(es). Points indicative of 4 replicates with SEM error bars. Two-way ANOVA was performed with Tukey correction for multiple comparisons, and asterisks indicate p<0.001 (***) and p<0.0001 (****).