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[Preprint]. 2024 Oct 3:2024.09.06.611729. [Version 3] doi: 10.1101/2024.09.06.611729

Figure 4. p300 differentially alters the conformational dynamics of PR-A and PR-B within the PR:SRC3:p300 complex.

Figure 4.

Top. Consolidated HDX plots of PR-B showing the differential HDX-MS comparisons within the plot to the left. Changes in deuterium uptake are represented by the rainbow plot shown at the bottom. Common PR domains are highlighted at the top: N-terminal domain (NTD, orange), DNA-binding domain (DBD, purple), Hinge (yellow), and ligand-binding domain (LBD, teal). Bottom. AlphaFold3.0 models of PR from the AF1 to LBD (amino acids 456–933 using PR-B numbering). HDX-MS overlays represent the same experiments as the consolidated views on the top. Each color represents the percent change in deuterium incorporation (Δ%D), following the scale shown at the bottom. Gray overlays indicate no significant changes and black indicates peptides not detected in the HDX-MS experiment.