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[Preprint]. 2024 Oct 3:2024.09.06.611729. [Version 3] doi: 10.1101/2024.09.06.611729

Figure 5. PR-A and PR-B differentially interact with SRC3 and are stabilized by p300 addition.

Figure 5.

Top. Consolidated differential HDX-MS results for SRC3, comparing the changes induced by PR-A and p300 binding in the presence and absence of PRE DNA. Middle. Consolidated HDX-MS plot of SRC3 exchange, with PR-B comparisons in the same order as PR-A. The motifs highlighted are the following: bHLH (orange), PAS (purple), LXXLL motifs (yellow), CREBBP Interaction domain (teal), and acetyltransferase domain (dark blue). Each color represents the percent change in deuterium incorporation (Δ%D), following the scale shown at the bottom.Gray overlays indicate no significant changes and black indicates peptides not detected in the HDX-MS experiment. Bottom. Selected deuterium uptake plots for peptides that contain LXXLL motifs 1, 2, and 3. The %D uptake indicates the percent deuterium uptake over time for the PR-A:SRC3 ± DNA and PR-A:SRC3:p300 ± DNA HDX experiments.