Figure 5. Loss of shank3^ex4−22 increases repetitive behavior and decreases exploratory behavior sex- and age-dependently.

(A, B) Individual (circles) and mean ± SEM (black bars) of the total duration of grooming time during open field exploration for each genotype at both ages (A) and further separated by sex (B). (C) Representative images of marble location after 30 min in the marble burying arena for one mouse of each genotype, age, and sex. (D-F) Mean ± SEM of the number of marbles buried after each 5 min period during the marble burying assay for each genotype at both ages (D) and further separated into males (E) and females (F) at each age. (B, H) Individual (circles) and mean ± SEM (black bars) of the number of marbles buried after 30 min for each genotype at both ages (G) and further separated by sex (H). (I-L) Individual animal (circles) and group mean ± SEM (black bars) of the total number of arm entries (I, J) and percent of alternations (K, L) in the Y-maze for each genotype at both ages (I, K) and further separated by sex (J, L). N = 20–31 mice/group for each genotype at each age and N = 10–18 mice/group for each sex within each genotype at each age*p<0.05 for post-hoc test between genotypes with a Bonferroni correction.