Sedimentation analysis of CAV9 uncoating after incubating cell-bound virus for 0, 1, or 3 h at 37°C. Prior to this incubation, 35S-labeled viruses (about 2.5 × 106 cpm) were allowed to adsorb to cells for 2 h at 18°C. Percentages of total label forming each peak (160S, 135S, and 80S) are shown.