Fig. 1. A schematic of Serial Lift-Out.

An illustration of the Serial Lift-Out method, exemplified by the process being performed on a C. elegans L1 larva embedded in an extracted volume of vitreous ice (blue). a, The extracted volume containing the larva. b,c, Schematic representation of the resulting Serial Lift-Out sections (b) and lamellae (c), respectively. d, Illustration of the double-sided attachment Serial Lift-Out procedure. The extracted volume shown in a is attached to the lift-out needle via a copper block adaptor and transferred to a rectangular mesh receiver grid. Several sections are shown, obtained by the repetition of the attachment of the bottom part of the volume to the grid bars and subsequent sectioning.