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. 2023 Dec 18;21(9):1684–1692. doi: 10.1038/s41592-023-02113-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, The C. elegans ribosome reconstructed by subtomogram averaging to a resolution of 6.9 Å (GSFSC). The density map is colored by local resolution. Note that protein α-helices and ribosomal RNA helices are clearly visible at this resolution. b, Four different ribosomal states were obtained through subtomogram classification: ribosome class 1 with occupied A, P and EF sites, ribosome class 2 with an occupied P site, ribosome class 3 with occupied A and P sites, ribosome class 4 with occupied A and EF sites. c, Tomographic slice of a tomogram collected from, what is likely, the ventral sublateral nerve cord with a touch-receptor neuron (PLML/R). Boxes highlight two different C. elegans microtubule assemblies made up of 11 and 15 protofilaments. d, Magnified tomogram section of the 11-protofilament microtubule from a hypodermal cell highlighted in c. e, Magnified tomogram section of the 15-protofilament microtubule from the touch-receptor cell highlighted in c. f, Subtomogram average of a focused region of the 11-protofilament microtubules resolved to 13 Å. Data presented in a and b stem from experiment 2; data presented in c–f stem from experiment 3.