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. 2024 Sep 13;13:17. doi: 10.1186/s13619-024-00200-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — UiPSCs can differentiate into lung progenitor cells in vitro. a Schematic diagram of lung progenitor cell differentiation. b Cell morphology at different time points during cell line differentiation into lung progenitor cells (scale: 200 μm). c Detection of lung progenitor cell markers (NKX2.1 and EPCAM) by immunofluorescence assay on day 15 after differentiation. Nuclear staining with DAPI (scale: 100 μm). d Flow cytometry detection of pulmonary progenitor cell markers (CD47 + &CD26- and EPCAM) on day 15 of differentiation. e Detection of lung precursor cell markers (NKX2.1 and EPCAM) in cells on day 15 of differentiation by qPCR