Figure 4.

GM47544 activated HL and LDLR by suppressing ApoC3 to enhance plasma TG clearance.

(A) Relative mRNA expression of GM47544 and ApoA1/C3/A4/A5 gene cluster measured by RT-qPCR.

(B) The ApoA1/C3/A4/A5 protein level was measured by western blot in control and GM47544-overexpressed AML-12 cells.

(C) Relative mRNA expression of GM47544 and ApoA1/C3/A4/A5 gene cluster measured by RT-qPCR.

(D) The ApoA1/C3/A4/A5 protein level was measured by western blot in the primary mouse hepatocytes transfected with empty vector (OE-Ctrl) and GM47544 overexpression plasmids (OE-GM47544).

(E) Relative mRNA expression of GM47544 and ApoA1/C3/A4/A5 gene cluster measured by RT-qPCR.

(F) The ApoA1/C3/A4/A5 protein level was measured by western blot in the livers of control and GM47544-overexpressed mice.

(G) Relative mRNA expression of GM47544 and ApoA1/C3/A4/A5 gene cluster measured by RT-qPCR.

(H) The ApoA1/C3/A4/A5 protein level was measured by western blot in the livers of control and GM47544-overexpressed Apoe^−/− mice.

(I) Plasma ApoC3 protein levels in control and GM47544-overexpressed mice.

(J) Plasma LPL activity stimulation in control and GM47544-overexpressed mice.

(K) Plasma ApoC3 protein levels in control and GM47544-overexpressed Apoe^−/− mice.

(L) Plasma LPL activity stimulation in control and GM47544-overexpressed Apoe^−/− mice.

Data are presented as mean ± SD in A, B, C, D, E, F, G, H, I, J, K, and L, with statistical significance determined using an unpaired two-tailed Student's t-test.