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. 2001 Jan;75(2):996–1003. doi: 10.1128/JVI.75.2.996-1003.2001

TABLE 1.

Early pathogenesis of AcMNPV-hsp70/lacZ in H. virescens and H. zeaa

Species Time (hpi) lacZ expressionb
Hemolymph BV
LacZ+ hemocytes
% Larvae Midgut Tracheae % Larvae Mean BV titer ± SE (PFU/ml) % Larvae % Cells
H. virescens 2 0 − − 0 0 0 0
4 87 + − 0 0 0 0
6 83 + + 0 0 0 0
8 95 + + 0 0 0 0
10 95 + + 0 0 0 0
12 100 + + 22 1.2 × 103 ± 4.1 × 102 0 0
14 100 + + 40 1.8 × 104 ± 1.4 × 104 20 0.01
16 100 + + 93 3.8 × 104 ± 7.4 × 103 36 0.01
H. zea 2 0 − − 0 0 0 0
4 90 + − 0 0 0 0
6 98 + + 0 0 0 0
8 100 + + 0 0 0 0
10 98 + + 0 0 0 0
12 100 + + 0 0 0 0
14 95 + + 0 0 0 0
16 98 + + 27 3.5 × 104 ± 1.5 × 104 12 0.01
a

Newly molted fourth-instar larvae were orally inoculated with 104 occlusions. Cohorts of 15 insects were bled and dissected at 2-h intervals from 0 to 16 hpi. 

b

Insects were processed for lacZ expression, and midgut and tracheal tissues were scored as present (+) or absent (−).