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. 2001 Jan;75(2):1004–1012. doi: 10.1128/JVI.75.2.1004-1012.2001

FIG. 3.

FIG. 3

Immunofluorescence analysis of G1 and G2 expression from transcripts from pSFV-GP (a to l) and of N expression from transcripts from pSFV-N (m). (a) At 6 h.p.t. with anti-TSWV; arrow indicates perinuclear signal. (b) At 21 h.p.t. with anti-TSWV. (c) At 6 h.p.t. with anti-TSWV. (d) Same cell treated with ER marker DiOC6. (e) Merge (double exposure) of c and d. (f) At 21 h.p.t. with anti-TSWV. (g) Same cell treated with Golgi marker WGA-FITC. (h) Merge of f and g. (i) At 21 h.p.t. with anti-TSWV. (j) Same cell treated with Golgi stack marker anti-p58. (k) Merge of i and j. (l) Expression in the presence of the glycosylation inhibitor tunicamycin at 21 h.p.t. with anti-TSWV. (m) At 21 h.p.t. with anti-N. Yellow areas in c, f, and i indicate overexposure of the film to clearly visualize additional (ER) localization elsewhere in the cell.