FIG. 8.

Viral protein accumulation (A) and immunoblot detection of cellular death factor processing (B) in mock (M)-, KOS1.1-, and d4-5-infected HEp-2 cells in the presence (+) or absence (−) of PAA (300 μg/ml). Whole-cell extracts prepared at 24 h p.i. were used for immunoblot analyses with anti-PARP and anti-caspase-3 antibodies or with anti-ICP27 and anti-ICP4 (IE proteins), anti-TK (E protein), anti-gD (L protein, γ₁), and anti-gC (L protein, γ₂) antibodies as described in Materials and Methods. 116 and 85 refer to full-length and processed PARP, respectively.