TABLE 1.

Features of the recombinant viruses used in this study

Virus	Source or reference	Mutation		Amino acid(s) affected	Viral DNA replication	Nuclear localization
		Type	Domain(s)
KOS1.1	24	None	None	None	Wild type	N, Nu, shuttlesb
d27-1	48	Deletion	All	1–512	Defective (49)	ND
d1-5	38	Deletion	Amino	13–153	NDa	ND
d1-2	50	Deletion	Amino	12–63	Defective (50)	N, shuttling reduced (37)
d2-3	This study	Deletion	Amino	64–109	ND	ND (38)
d3-4	36	Deletion	Amino	109–138	ND	N>C (38)
d4-5	36	Deletion	Amino	139–153	ND	N, not Nu (38)
d5-6	38	Deletion	Amino	154–173	ND	ND
d6-7	This study	Deletion	Amino	174–200	ND	ND
M11	49	Point, RD to LE	Carboxy	340, 341	Wild type (49)	N, shuttling reduced (37, 38)
M15	49	Point, PG to LE	Carboxy	465, 466	Wild type (48)	N, no shuttling (37)
M16	49	Point, C to L	Carboxy	488	Wild type (49)	N, shuttling reduced (37)
n504R	48	Nonsense	Carboxy	505–512	Wild type (48, 49)	N diffuse (48, 50), shuttle (37)
n406R	48	Nonsense	Carboxy	407–512	Defective (49)	N punctate, not Nu (48, 51)
n263R	48	Nonsense	Carboxy	264–512	Defective (49)	N, preferentially Nu (48, 51)
n59R	48	Nonsense	Carboxy	60–512	Defective (49)	Protein not detected (45, 51)

^aND, not determined. 

^bN, nuclear; Nu, nucleolar.