TABLE 2.

Comparison of apoptosis in HEp-2 cells and growth properties in Vero cell lines

Virus	% Apoptotic cells	% PARP processing	HEp-2 cell phenotypec	Vero cell titer (PFU/ml)	V27 cell titer (PFU/ml)	Plaquing ratio (Vero/V27)	Functional region(s) affected
KOS1.1	8	21	NA	4.8 × 108	3.0 × 108	1.6	None
d27-1	89	96	A	<2.0 × 103	4.0 × 108	<5.0 × 10−6	All
d1-5	78	93	A	<2.0 × 103	4.0 × 108	<5.0 × 10−6	NES, acidic domain, NLS, NuLS, RGG box
d1-2	58	84	A	<2.0 × 104	2.8 × 108	<7.1 × 10−5	NES, acidic domain
d2-3	14	30	NA	4.6 × 108a	8.0 × 108	5.8 × 10−1	*d
d3-4	18	54	NA	7.8 × 107b	4.4 × 108	1.8 × 10−1	NLS, NuLS
d4-5	17	28	NA	9.0 × 105b	1.9 × 108	4.7 × 10−3	NuLS, RGG box
d5-6	12	46	NA	3.0 × 108	8.0 × 108	3.8 × 10−1	*
d6-7	9	31	NA	2.2 × 108	6.0 × 108	3.7 × 10−1	*
M11	36	71	PA	4.2 × 103	1.4 × 108	3.0 × 10−5	Activation/repression domain
M15	56	86	A	<2.0 × 102	1.8 × 108	<1.1 × 10−6	Activation/repression domain
M16	31	58	PA	4.0 × 102	1.0 × 108	4.0 × 10−6	Activation/repression domain, putative zinc finger
n504R	42	80	PA	<2.0 × 102	8.8 × 107	<2.3 × 10−6	Activation/repression domain, putative zinc finger
n406R	35	54	PA	<2.0 × 102	1.3 × 108	<1.5 × 10−6	Activation/repression domain, putative zinc finger
n263R	69	91	A	<2.0 × 102	1.0 × 108	<2.0 × 10−6	Activation/repression domain, putative zinc finger
n59R	78	94	A	<2.0 × 102	1.6 × 108	<1.3 × 10−6	Acidic domain, NLS, NuLS, RGG box, putative zinc finger, activation/repression domain

^aSmall plaques. 

^bMinute plaques. 

^cNA, nonapoptotic; A, apoptotic; PA, partially apoptotic. 

^d∗, no function defined.