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. 2001 Jan;75(2):1013–1030. doi: 10.1128/JVI.75.2.1013-1030.2001

TABLE 2.

Comparison of apoptosis in HEp-2 cells and growth properties in Vero cell lines

Virus % Apoptotic cells % PARP processing HEp-2 cell phenotypec Vero cell titer (PFU/ml) V27 cell titer (PFU/ml) Plaquing ratio (Vero/V27) Functional region(s) affected
KOS1.1 8 21 NA 4.8 × 108 3.0 × 108 1.6 None
d27-1 89 96 A <2.0 × 103 4.0 × 108 <5.0 × 10−6 All
d1-5 78 93 A <2.0 × 103 4.0 × 108 <5.0 × 10−6 NES, acidic domain, NLS, NuLS, RGG box
d1-2 58 84 A <2.0 × 104 2.8 × 108 <7.1 × 10−5 NES, acidic domain
d2-3 14 30 NA 4.6 × 108a 8.0 × 108 5.8 × 10−1 *d
d3-4 18 54 NA 7.8 × 107b 4.4 × 108 1.8 × 10−1 NLS, NuLS
d4-5 17 28 NA 9.0 × 105b 1.9 × 108 4.7 × 10−3 NuLS, RGG box
d5-6 12 46 NA 3.0 × 108 8.0 × 108 3.8 × 10−1 *
d6-7 9 31 NA 2.2 × 108 6.0 × 108 3.7 × 10−1 *
M11 36 71 PA 4.2 × 103 1.4 × 108 3.0 × 10−5 Activation/repression domain
M15 56 86 A <2.0 × 102 1.8 × 108 <1.1 × 10−6 Activation/repression domain
M16 31 58 PA 4.0 × 102 1.0 × 108 4.0 × 10−6 Activation/repression domain, putative zinc finger
n504R 42 80 PA <2.0 × 102 8.8 × 107 <2.3 × 10−6 Activation/repression domain, putative zinc finger
n406R 35 54 PA <2.0 × 102 1.3 × 108 <1.5 × 10−6 Activation/repression domain, putative zinc finger
n263R 69 91 A <2.0 × 102 1.0 × 108 <2.0 × 10−6 Activation/repression domain, putative zinc finger
n59R 78 94 A <2.0 × 102 1.6 × 108 <1.3 × 10−6 Acidic domain, NLS, NuLS, RGG box, putative zinc finger, activation/repression domain
a

Small plaques. 

b

Minute plaques. 

c

NA, nonapoptotic; A, apoptotic; PA, partially apoptotic. 

d

∗, no function defined.