TABLE 2.
Comparison of apoptosis in HEp-2 cells and growth properties in Vero cell lines
Virus | % Apoptotic cells | % PARP processing | HEp-2 cell phenotypec | Vero cell titer (PFU/ml) | V27 cell titer (PFU/ml) | Plaquing ratio (Vero/V27) | Functional region(s) affected |
---|---|---|---|---|---|---|---|
KOS1.1 | 8 | 21 | NA | 4.8 × 108 | 3.0 × 108 | 1.6 | None |
d27-1 | 89 | 96 | A | <2.0 × 103 | 4.0 × 108 | <5.0 × 10−6 | All |
d1-5 | 78 | 93 | A | <2.0 × 103 | 4.0 × 108 | <5.0 × 10−6 | NES, acidic domain, NLS, NuLS, RGG box |
d1-2 | 58 | 84 | A | <2.0 × 104 | 2.8 × 108 | <7.1 × 10−5 | NES, acidic domain |
d2-3 | 14 | 30 | NA | 4.6 × 108a | 8.0 × 108 | 5.8 × 10−1 | *d |
d3-4 | 18 | 54 | NA | 7.8 × 107b | 4.4 × 108 | 1.8 × 10−1 | NLS, NuLS |
d4-5 | 17 | 28 | NA | 9.0 × 105b | 1.9 × 108 | 4.7 × 10−3 | NuLS, RGG box |
d5-6 | 12 | 46 | NA | 3.0 × 108 | 8.0 × 108 | 3.8 × 10−1 | * |
d6-7 | 9 | 31 | NA | 2.2 × 108 | 6.0 × 108 | 3.7 × 10−1 | * |
M11 | 36 | 71 | PA | 4.2 × 103 | 1.4 × 108 | 3.0 × 10−5 | Activation/repression domain |
M15 | 56 | 86 | A | <2.0 × 102 | 1.8 × 108 | <1.1 × 10−6 | Activation/repression domain |
M16 | 31 | 58 | PA | 4.0 × 102 | 1.0 × 108 | 4.0 × 10−6 | Activation/repression domain, putative zinc finger |
n504R | 42 | 80 | PA | <2.0 × 102 | 8.8 × 107 | <2.3 × 10−6 | Activation/repression domain, putative zinc finger |
n406R | 35 | 54 | PA | <2.0 × 102 | 1.3 × 108 | <1.5 × 10−6 | Activation/repression domain, putative zinc finger |
n263R | 69 | 91 | A | <2.0 × 102 | 1.0 × 108 | <2.0 × 10−6 | Activation/repression domain, putative zinc finger |
n59R | 78 | 94 | A | <2.0 × 102 | 1.6 × 108 | <1.3 × 10−6 | Acidic domain, NLS, NuLS, RGG box, putative zinc finger, activation/repression domain |
Small plaques.
Minute plaques.
NA, nonapoptotic; A, apoptotic; PA, partially apoptotic.
∗, no function defined.