Fig. 1.

Single-Cell RNA-Seq revealed aortic transcriptional landscape and melatonin related EC-VSMC communication in CVD and healthy controls. (A)Schematic diagram of the experimental design. (B) Uniform Manifold Approximation and Projection (UMAP)representation of aligned gene expression data in single cells extracted from control (n = 15) and CVD (n = 13) aortas. (C) Violin plots for gene expression of melatonin receptors in cell populations of aorta from patients with CVD and controls. (D) Cell-specific expression of genes with known coding mutations associated with CVD. (E) Cell-Cell communication analysis of cells between CVD patients and control volunteers by cellphone DB.(F) GO analysis of top 50 differentially expressed genes in patients with CVD and controls (ordered by decreasing p value).