Fig. 2.

Exosomes transferred from ECs to VSMCs. (A) Schematic diagram of transwell co-culture system. VSMCs were co-cultured with ECs transfected with FAM-labeled miR-126–3p or FAM alone. (B) Fluorescence signal detection in VSMCs within co-culture system by confocal microscope (scale bar = 20 μm). (C) The expression level of miR-126–3p in co-cultured VSMCs by qRT-PCR. (D) Morphologic detection of exosomes by laser electron microscope (scale bar = 200 nm). (E) Particle diameter and concentration of exosomes detected by Malvern's NanoSight NS300. (F) The protein expression level of CD9, CD81, TSG101 and Grp94 detected by Western blot. (G) VSMCs were treated with PKH26 (red) labeled exosomes, and the fluorescence signal was detected by inverted fluorescence microscope (scale bar = 100 μm). (H) VSMCs co-cultured with ECs treated with DMSO or GW4869 prior to transfection with FAM labeled miR-126–3p or FAM alone. Fluorescence signal detection in VSMCs by confocal microscope (scale bar = 20 μm). Three independent experiments were performed, and representative images were shown. Data were shown as mean ± SEM. ***p < 0.001.