FIG. 2.

(A) Schematic arrangement of the DNA sequence of HSV-1 and the ICP27 gene. (Top) EcoRI restriction fragments of HSV-1 DNA were designated alphabetically according to their sizes. (Middle) HSV-1 EcoRI restriction fragment B containing genes from UL52 to ICP4. (Bottom) Schematic representation of the ICP27 coding region displaying the putative NES, the essential acidic domain, and other motifs. (B) Result of marker transfer experiments. After cotransfection of DNA fragment plasmids in conjunction with the WT KOS infectious DNA and a subsequent 2-day incubation, samples were frozen at −80°C, thawed, and analyzed by plaque formation assay in the presence or absence of LMB. Alphabetical letters represent the EcoRI restriction fragments, and I-V, P-V, and V-I represent the 8.5-kb EcoRI-EcoRV, 5.9-kb PstI-EcoRV, and 12.9-kb EcoRV-EcoRI fragments contained within the B fragment derived from the resistant mutant, Cl1, respectively. The DNA designated ACT contains the same sequence as the WT, 5.9-kb PstI-EcoRV fragment with a mutation (ATG [Met] to ACT [Thr]) at the 50th residue of ICP27.