FIGURE 3.
LC–MS data showing potential for JMJD7‐catalyzed (2 μM) hydroxylation of DRG1 peptides (10 μM) in the presence of 2OG (10 μM), FAS (10 μM) and LAA (100 μM). (a) DRG1‐Lys, (b) DRG1‐D‐Lys, (c) DRG1‐Orn, (d) DRG1‐hLys, (e) DRG1‐LysMe, (f) DRG1‐HNle, (g) DRG1‐Ahp, (h) DRG1‐CysNH2, (i) DRG1‐CysNHMe, (j) DRG1‐LysN, (k) DRG1‐LysE, (l) DRG1‐nArg, (m) DRG1‐hGln, (n) DRG1‐4pyrA, (o) DRG1‐βhLys, (p) DRG1‐LysCMe, (q) DRG1‐LysNMe, and (r) DRG1‐Abg. See Figure 4 for description of the fragmented products resulting from reaction of DRG1‐CysNH2 and DRG1‐LysN.