Figure 3.

PAGE analysis of in vitro transcription by T7 RNA polymerase using template2. In vitro transcription was carried out as described in Materials and Methods in the presence of four NTPs (lanes 3–6, for 10, 30, 60 and 180 min, respectively); GTP and ATP plus thioUTP and thioCTP (lanes 7–10, for 10, 30, 60 and 180 min, respectively); GTP and ATP plus 2′-NH₂dUTP and 2′-NH₂dCTP (lanes 11–14, for 10, 30, 60 and 180 min, respectively), and GTP and ATP plus 2′-FdUTP and 2′-FdCTP (lanes 15–18, for 10, 30, 60 and 180 min, respectively). Lanes 1 and 2 are sequence marker obtained by treatment of RNA30 with 50 mM aq. Na₂CO₃ (pH 9.0) and RNase T1, respectively.