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. 2005 May 24;33(9):2942–2951. doi: 10.1093/nar/gki578

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© The Author 2005. Published by Oxford University Press. All rights reserved

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(A) PAGE analysis of reverse transcription by Superscript™ II (RNase H⁻). Reverse transcription was carried out as described in Materials and Methods in the presence of labeled primer only (lane 1); labeled primer and RNA59 as a template (lane 2); labeled primer and thioRNA59 as a template (lane 3). (B) Electropherogram of DNA-sequencing fragments.