TABLE 1.

Two-hybrid interactors with the Hsp82(E33A)-BD bait fusion

ORFc	Gene	Inta	Function of gene productb
Known or putative Hsp90 system cochaperones
YDR214w	AHA1	2	Nonessential cochaperone which binds the middle domain of Hsp90 (36) and activates the Hsp90 ATPase (44)
YDR168w	CDC37	1	Essential Hsp90 system cochaperone; believed to target protein kinases to the Hsp90 chaperone machine (32)
YBR155w	CNS1	1	Essential Hsp90 system cochaperone
YKL117w	SBA1	2	Nonessential Hsp90 system cochaperone; binds the N-terminal Hsp90 domain in mature Hsp90-client complexes, probably assisting client protein release (69)
YOR027w	STI1	1	Nonessential stress-induced protein that interacts with both Hsp70 and Hsp90 through its TPR domains (72)
YOR035c	SHE4	3	Nonessential protein required for mother cell-specific expression of homothallic switching endonuclease; equivalent of the UNC45 myosin-specific chaperone, of metazoans (2); though fungal forms of She4p lack the Hsp90-interacting TPR domain of UNC45 (16), the yeast She4p still binds Hsp90 (S. H. Millson, unpublished data)
YPL106c	SSE1	1	Nonessential form of Hsp110, a member of a subgroup of Hsp70 family chaperones (56)
YCR060w	TAH1	2	Nonessential protein with TPR repeats and similarity to Sti1p
YNL064c	YDJ1	2	DnaJ homolog; activator of the ATPase of Hsp70
Other stress proteins, chaperones, chaperonins
YDL143w	CCT4	1	Component of chaperonin-containing T complex (TCP ring complex, TRiC), required for the assembly of actin and tubulins in vivo
YOL053c-a	DDR2	1	Stress protein induced by DNA damage, heat shock, osmotic shock, and oxidative stress
YOR020c	HSP10	1	Mitochondrial chaperonin that cooperates with Hsp60p; homolog of E. coli GroES
YDR171w	HSP42	2	Heat shock protein with similarity to Hsp26p; involved in restoration of the cytoskeleton during mild stress
YLR259c	HSP60	1	Mitochondrial chaperonin that cooperates with Hsp10p; homolog of E. coli GroEL
Cytoskeleton
YFL039c	ACT1	2	Actin, critical for cell polarization, endocytosis, and other cytoskeletal functions
YNL079c	TPM1	2	Tropomyosin, coiled-coil protein localized to actin cables
YHR107c	CDC12	2	Component of 10-nm filaments of mother-bud neck (septin); involved in cytokinesis.
Protein kinases
YDR283c	GCN2	2	Kinase that phosphorylates translation initiation factor eIF2; known Hsp90 client protein (10)
YLR113w	HOG1	2	MAP kinase of the high-osmolarity signal transduction pathway
YGR040w	KSS1	1	MAP kinase involved in signal transduction pathways that control filamentous growth and pheromone response
YHR030c	SLT2	2	MAP kinase of the cell wall integrity (hypotonic stress-activated) pathway
YLR362w	STE11	1	MAP kinase kinase kinase of the Hog1p and Kss1p MAP kinase pathways; known Hsp90 client protein (31)
YHR135c	YCK1	1	Casein kinase I isoform
Known or putative transcriptional activators
YLR256w	HAP1	2	Heme-responsive Zn(2)-Cys(6) binuclear cluster domain transcription factor; redox-sensing regulator of gene expression; known Hsp90 client protein (29)
YBL005w	PDR3	2	Zn(2)-Cys(6) binuclear cluster domain transcription factor; regulator of genes of pleiotropic drug resistance
YCR029c-a	RIM1	1	Zinc finger single-stranded DNA-binding protein; required for mitochondrial genome maintenance
YKR064w	YKR064w	1	Zn(2)-Cys(6) binuclear cluster domain protein with similarity to transcription factors
Known or putative regulators of transcriptional silencing or telomere organizations
YJL076w	ESC5	2	Involved in chromatin silencing, has similarity to Tof2p
YMR263w	SAP30	2	Subunit of a histone deacetylase complex
YDL042c	SIR2	1	NAD-dependent histone deacetylase; involved in maintenance of silencing at HMRd, HMLd, and telomeres
YOR229w	WTM2	1	Transcriptional modulator protein involved in meiotic regulation and silencing
Protein import or export from the nucleus
YJR074w	MOG1	2	Involved in nuclear protein import; potential Cdc28p substrate
YJR132w	NMD5	3	Beta-karyopherin; involved in nuclear import
YGR119c	NUP57	2	Nuclear pore protein (nucleoporin) that acts in a complex with Nic96p, Nup49p, and Nsp1p
Ras superfamily small GTPases
YOR101w	RAS1	1	GTP-binding protein involved in regulation of cyclic AMP pathway; homolog of mammalian proto-oncogene ras
YML001w	YPT7	2	GTP-binding protein of the Rab family (Ras superfamily) with a role in protein transport between endosome-like compartments
YER031c	YPT31	2	GTP-binding protein of the Rab family (Ras superfamily) required in the secretory pathway at the stage of formation of trans-Golgi vesicles
YNL093w	YPT53	2	GTP-binding protein of the Rab family (Ras superfamily) involved in endocytosis and transport of proteins to the vacuole
Other proteins of vesicle trafficking
YLR078c	BOS1	2	Synaptobrevin (v-SNARE) homolog involved in endoglasmic reticulum (ER)-to-Golgi transport
YAL042w	ERV46	2	Protein localized to Sec31p-coated vesicles
YOR036w	PEP12	2	Syntaxin homolog (t-SNARE) involved in Golgi-to-vacuole transport
YKL006c-a	SFT1	2	v-SNARE required for vesicle traffic between Golgi compartments
YOL018c	TLG2	1	Syntaxin homolog (t-SNARE) involved in endocytosis and maintenance of resident proteins in the trans-Golgi network
YDR084c	TVP23	2	Integral membrane protein localized to vesicles along with the v-SNARE Tlg2p
YJR044c	VPS55	2	Vacuolar protein sorting; involved in Golgi-to-vacuole targeting
YGL161c	YIP5	2	Protein that interacts with Rab GTPases; possible role in vesicle-mediated transport
Known or putative membrane transporters
YBR132c	AGP2	2	General amino acid permease
YGR289c	AGT1	2	General alpha-glucoside permease with similarity to maltose permeases and other members of the sugar permease family
YPR128c	ANT1	1	Protein with similarity to ADP/ATP carrier proteins and members of the mitochondrial carrier family (MCF)
YNL065w	AQR1	2	Member of major facilitator superfamily (MFS) multidrug resistance (MFS-MDR) protein family
YER119c	AVT6	3	Vacuolar transporter; exports aspartate and glutamate from the vacuole
YDR046c	BAP3	3	Branched-chain amino acid permease, valine transporter
YOL137w	BSC6	2	MFS protein of unknown function.
YBR290w	BSD2	2	Copper ion transporter of the ER; metal homeostasis
YEL063c	CAN1	2	Permease for arginine, lysine, and histidine
YJR152w	DAL5	2	Allantoate and ureidosuccinate permease, MFS superfamily member
YNL125c	ESBP6	2	Protein with similarity to mammalian monocarboxylate transporters MCT1 and MCT2
YAL022c	FUN26	3	Nucleoside transporter with broad nucleoside selectivity
YHR094c	HXT1	2	Low-affinity hexose transporter and member of sugar permease family; induced by high glucose
YHR096c	HXT5	2	Strong similarity to hexose transporters; member of sugar permease family
YFL011w	HXT10	2	Hexose transporter; member of sugar permease family
YNR072w	HXT17	2	Similarity to hexose transporters, including Hxt13p, Hxt16p, Hxt6p, Hxt7p, and Lgt1p
YNL268w	LYP1	2	Lysine-specific permease, high affinity
YDL247w	MPH2	2	Alpha-glucoside permease
YGR055w	MUP1	2	High-affinity methionine permease
YHL036w	MUP3	2	Low-affinity methionine permease
YJL117w	PHO86	3	Phosphate permease
YOR348c	PUT4	2	Proline and gamma-aminobutyrate permease
YDL199c	YDL199c	3	Similarity to members of the sugar permease family
YIL120w	YIL120w	2	Member of MFS-MDR protein family
YKL174c	YKL174c	2	Similarity to Hnm1p and other permeases
YMR155w	YMR155w	2	Unknown function, MFS superfamily member
YPL264c	YPL264c	2	Unknown function, MFS superfamily member
YPR003c	YPR003c	2	Member of the sulfate permease subfamily of MFS superfamily
Miscellaneous
YCR048w	ARE1	2	Acyl coenzyme A (CoA):sterol acyltransferase (sterol-ester synthetase)
YNR019w	ARE2	2	Acyl-CoA:sterol acyltransferase (sterol-ester synthetase)
YHR018c	ARG4	2	Argininosuccinate lyase; arginine biosynthesis
YPR201w	ARR3	2	Arsenic resistance protein
YJL170c	ASG7	2	Protein expressed only in cells of mating type a
YBR128c	ATG14	2	Required for autophagy
YPR171w	BSP1	2	Binding protein of synaptojamin polyphosphoinositide phosphate domain
YLR438w	CAR2	2	Ornithine aminotransferase
YIL003w	CFD1	3	Putative P-loop ATPase localized in cytoplasm
YLL018c	DPS1	2	Aspartyl-tRNA synthetase, cytosolic
YLR284c	EHD1	1	Enoyl-CoA hydratase, peroxisomal
YHR123w	EPT1	2	sn-1,2-Diacylglycerol ethanolaminephosphotransferase
YFL022c	FRS2	1	Phenylalanyl-tRNA synthetase, beta subunit, cytoplasmic
YEL042w	GDA1	1	Guanosine diphosphatase of Golgi membrane
YHR183w	GND1	3	6-Phosphogluconate dehydrogenase
YPL059w	GRX5	1	Mitochondrial glutaredoxin
YJR069c	HAM1	1	Protein controlling 6-N-hydroxylaminopurine sensitivity and mutagenesis
YDR224c	HTB1	2	Histone H2B
YDL125c	HNT1	2	Member of the HIT family of Zn-binding proteins and the histidine triad nucleotide-binding protein family
YPR033c	HTS1	1	Histidyl-tRNA synthetase, mitochondrial and cytoplasmic
YOL065c	INP54	2	Inositol polyphosphate 5-phosphatase
YOL101c	IZH4	2	Membrane protein involved in zinc metabolism
YDL131w	LYS21	2	Homocitrate synthase, involved in lysine metabolism
YDL078c	MDH3	1	Malate dehydrogenase, peroxisomal
YKL001c	MET14	2	Adenosine-5′-phosphosulfate 3′-phosphotransferase; sulfate assimilation pathway
YIL014w	MNT3	2	Alpha-1,3-mannosyltransferase
YNL110c	NOP15	2	Constituent of 66S preribosomal particles
YGL055w	OLE1	3	Stearoyl-CoA desaturase (delta-9 fatty acid desaturase); synthesis of unsaturated fatty acids
YOL147c	PEX11	2	Peroxisomal biogenesis
YBR035c	PDX3	2	Pyridoxine (pyridoxamine) phosphate oxidase
YHR037w	PUT2	1	Delta-1-pyrroline-5-carboxylate dehydrogenase
YOR347c	PYK2	1	Pyruvate kinase, glucose-repressed isoform
YCR106w	RDS1	2	Regulator of drug sensitivity
YOL120c	RPL18A	2	Ribosomal protein L18A
YPL152w	RRD2	1	Protein involved in rapamycin sensitivity
YDL244w	THI13	1	Thiamine synthesis
YER100w	UBC6	2	Ubiquitin-conjugating enzyme anchored in the ER membrane, with catalytic activity facing into cytoplasm
YBR273c	UBX7	2	Ubiquitin regulatory X domain-containing protein; interacts with Cdc48p
YKL035w	UGP1	2	UDP-glucose pyrophosphorylase (UTP-glucose-1-P uridylyltransferase) (UGPase)
YBR130c	SHE3	2	Required for mother cell-specific expression of HO
YER118c	SHO1	3	Osmosensor in the Hog1p MAP kinase; high-osmolarity signal transduction pathway
YML066c	SMA2	1	Spore membrane assembly
YJR159w	SOR1	1	Sorbitol dehydrogenase
YMR101c	SRT1	1	cis-prenyltransferase involved in dolichol synthesis
YIR011c	STS1	1	Protein that when overexpressed restores protein transport and rRNA stability to a sec23 mutation
YPL048w	TEF3	1	Translation elongation factor EF-1gamma
YNL131w	TOM22	2	Component of mitochondrial outer membrane receptor complex; required for protein import and cell viability
YOR332w	VMA4	2	Vacuolar H-ATPase hydrophilic (27-kDa) subunit
YOR083w	WHI5	2	Cell size regulation of passage through start and commitment to cell division
YLR070c	XYL2	2	Xylitol dehydrogenase
YJL114w	YJL114w	1	TyA Gag protein; main structural constituent of virus-like particles

^aStrength of interaction. 1, 2, and 3 indicate growth of the cells containing this interactor and the Hsp82(E33A)-BD bait to 4 mM, 8 mM, or 20 mM 3-AT, respectively.

^bListed where a function has been assigned. The following are the ORFs encoding interactors of unknown function, with the corresponding gene name, where assigned, and the interaction strength of the encoded interactor in parentheses: YAL049c (1); YBR220c (2); YBR227c (1); YCL056c (2); YCR101c (3); YDL012c (2); YDL015c (TSC13) (2); YDL133w (1); YDL180w (1); YDL246c (SOR2) (1); YDL248c (COS7) (2); YDR051c (1); YDR281c (PHM6) (2); YDR319c (2); YDR412w (1); YER156c (2); YHR036w (2); YGR295c (COS6) (2); YIL041w (3); YIL146c (ECM37) (2); YIR030c (DCG1) (1); YIR042c (2); YJL060w (BNA3) (2); YJR083c (ACF4) (3); YJR134c (2); YKL008c (LAC1) (2); YKL069w (2); YKR030w (GMH1) (3); YKR044w (UIP5) (2); YKR067w (1); YLR036c (1); YLR404w (2); YML131w (2); YML132w (COS3) (2); YMR159c (SAP18) (2); YNL100w (1); YNL194c (1); YNL326c (2); YOL026c (MIM1) (1); YOR097c (2); YPL014w (1); YPL109c (1); YPL156c (PRM2) (1); YPL186c (UIP4) (1); YPL188w (POS5) (1); YPL199c (2); YPL246c (RBD2) (1); YPL257w (2); YPR089w (3); YPR115w (2); YPR127w (2); YPR148c (1).

^cORF, open reading frame.

^dMating type cassettes on chromosome 3.