FIG. 3.

Northern blot analysis of Rim101p-repressed genes. Wild-type and mutant S. cerevisiae strains, as indicated above each lane, were grown in YPD medium and then shifted to YPD medium, pH 8, for approximately 4 h before RNA was isolated. Each lane contained 20 μg total RNA; lanes 1 to 10 and 11 to 20 show two different Northern blots prepared in parallel. The blots were probed for NRG1 or SMP1, as indicated on the left of each panel, and then stripped and probed for the loading control, ENO1.