FIG. 5.

Northern blot analysis of Snf7p-responsive genes. (A) Wild-type and mutant S. cerevisiae strains, as indicated above each lane, were grown in YPD medium and then shifted to YPD medium, pH 8, for approximately 4 h before RNA was isolated. Each lane contained 20 μg of total RNA; lanes 1 to 10 and 11 to 20 show two different Northern blots prepared in parallel. The blots were probed for PRY1 or ASN1, as indicated on the left of each panel, and then stripped and probed for the loading control, ENO1. (B) Probe intensities relative to the wild type were normalized for loading against the ENO1 signal for the Northern blots in panel A.